LOCUS       UNVERIFIED.        7041 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pGEM-T MCD KO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    UNVERIFIED
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Feng J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen 
            University, Department of Dermatology, 107 West Yanjiang Rd, 
            Guangzhou, Guangdong 510120, China
REFERENCE   2  (bases 1 to 7041)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7041)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2018) Sun Yat-sen Memorial Hospital, Sun Yat-sen University,
            Department of Dermatology, 107 West Yanjiang Rd, Guangzhou, 
            Guangdong 510120, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
            GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
FEATURES             Location/Qualifiers
     source          1..7041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4166..4184)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4202..4218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4226..4242)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4250..4280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4295..4316)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4604..5192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5366..6223)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6224..6328)
                     /label=AmpR promoter
     rep_origin      complement(6406..6861)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7002..7018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7025..7041
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 accattcagt ccagcacata caaggtcgta caatcaacag agagatgaag aaaattcatc
      121 tctaattttc agtgctttca acatggttca aaaggttaaa cctcgtgttc atactattga
      181 ggagacgttc ggtgtaccat caaggcacaa agagacattt ctccggatga ttcaagattt
      241 cttggaactt ggttattcca tacacagcaa gactattgca tgtgcgcaat atggagttcc
      301 gcaaatgcga cgacgtcttt tcatcattgc ggctgggtga gtttgaatgt catacctacg
      361 gccagatcta tacttacgta ctattaggcc aggggaaagg ctgcctacct tcccaactcc
      421 tactcatggg ccggacctat taccttatgt cacgatcaag gatgcgataa gcaacatacc
      481 ccgtctagct gataatcacg gccctgacaa tgtcgctttt caagatggta ggacccgggc
      541 tccgtacgac gagaacacgc tcgccaaaac aataacgtgc ggtggcggag acggaaacta
      601 ccatccaagt ggtcttagac ccttcactat ccgtgaattg gcttgcttac agactttccc
      661 cgtgcagtat cgattctcaa cagcctatgc aaagaagcaa gtcggcaact cggttccgcc
      721 tcgtctcgca gaggttattt atcgagctgc tatcaagtca cttcaggaga ctgacgacaa
      781 ggagtgcagg gagccaatcg taattgattg agctagtcat tgggcattca gcatgtcaag
      841 acaatatgat ggaagcgaat gttaactcgt gtggtggtcc attgagtatg aggctatagt
      901 ttggatggag atgttgttgt ggaatcttcc gaagaccggc ggatctacgc gaatcgtcgt
      961 ttatctgggg aaggtttatc aggtcaaaaa tacgttatca tactctgtac ttcacgctat
     1021 tccccacatg ttttggggca acgagttgag ggcctcttct ttttcgtttg cgtttacgga
     1081 gtagaaaaaa gggaaattga ttaatgaata atctcgagat atgctcaaag tcaattgaaa
     1141 tgtttgataa ttgctttttc cctctccgta ttcaggtaca acccccgtgg cagagacgtt
     1201 cctggtatgt aatgatatgt ttcggttggc cgcggctgac accacatccc caggtgggac
     1261 ccatactacg gagtagatta gggccaaaag tacggtaaat tacaccggaa atacacgata
     1321 cgcgtactga cgagctcgac gtcaagctga cggagagaag gaaagcttcc gtttccccca
     1381 gcctcggtca acaactctct ccctccttcc cacctctctt tcttcctgtg gcgctattca
     1441 attctccgat ttcaggtcta tatctatcca ttgacttgtc caacgacaat ccgtcgtttg
     1501 tttttgttcc tcccgcagac aatgctctct atcctggtgg caggcgtcaa gtacccagag
     1561 gcagcagcgg gcttaggagc ggcctgggtt gttctccgca ccctctacat gctgggctat
     1621 atttatagcg acaagccgaa cggcaccggc aggtacaatg gttcgctgta cttgcttgcg
     1681 caagcgggtc tttggggatt gagcgcattt ggtgttgcaa aggatttgat gtaaatgtag
     1741 tcgacatctt agcacagagg ggagagttga taaaatgtgg tctgtttgaa tgatagtcgg
     1801 gttcgtgacc tatattcgtg atagtggaga taggtctgcg cctatcttat cgggccggag
     1861 caaaaattcc accgcagcgg ggtgagtttt cgttatacag ccatcccact tccagcttca
     1921 aattgtcagt ttaatccagc ccaattcaat cattggagaa ccgccatcat gtcttcgaag
     1981 tcccacctcc cctacgcaat tcgcgcaacc aaccatccca accctttaac atctaaactc
     2041 ttctccatcg ccgaggagaa gaaaaccaac gtcaccgtct ccgcagacgt tactacttcc
     2101 gccgagctcc tcgatcttgc tgaccgtaca tcctgcacca atgcccctcc aggataacaa
     2161 atagctgatg cgtagtgagt acaggcctag gcccctatat cgcagttctg aaaacccaca
     2221 tcgacatcct caccgatctc accccgtcga ccctttcctc gctccaatcc ctcgcgacaa
     2281 agcacaactt cctcatcttt gaggaccgca agttcatcga catcggcaac accgtgcaaa
     2341 agcagtacca cggtggcgct ctccgcatct ccgaatgggc acacatcatc aactgcgcca
     2401 tcctgccggg cgaagggatc gtcgaggccc tcgcacagac aaccaagtct cctgacttta
     2461 aagacgcgaa tcaacgaggt ctcctgattc ttgccgagat gacgagtaag ggatctcttg
     2521 cgacagggga gtcacaggca cgctcggttg agtacgcgcg gaagtataag gggtttgtga
     2581 tgggattcgt gagtacaagg gcgttgagtg aggtgctgcc cgaacagaaa gaggagagcg
     2641 aggattttgt cgtctttacg actggggtga atctgtcgga taagggggat aagctggggc
     2701 agcagtatca gacacctggg tcggcggttg ggcgaggtgc ggactttatc attgcgggta
     2761 ggggcatcta taaggcggac gatccagtcg aggcggttca gaggtaccgg gaggaaggct
     2821 ggaaagctta cgagaaaaga gttggacttt gagtgtgagt ggaaatgtgt aacggtattg
     2881 acgcagcgag atgtgtatgt ttgttaatat agagactagt ttagagccaa tgataagatc
     2941 agtgatcaag acagttcatt aagcacatcg ttgacaatat atctgggtat cgtagtatat
     3001 tctatagtgg gtatattcaa aacgagagtc aaacgctgcc atctatccct tcacgccgtt
     3061 attttccgct tcattgctcc ccacaaccga gtctcctctt gtaaactcag cattcacctc
     3121 tctgtcatta ttagtcgcat gtttatgggc cctgttgccc ccctcaggat accccgtatc
     3181 tctccagacc tcccaaatct tcgcgccgcg ctgcaaactc gacacattct cccaaatgag
     3241 acaaaacacg accaacgcca taatcacgga gagtgtctct gtcaaattca acgatttcgc
     3301 aagcggtagt aacaccaata tcagccctac tacaggccgc attatgattc ggaaatgttt
     3361 cggaagtaga agcttgcagt cgccgtcgtt ggaggagtag agcagcgcca tggcgtagag
     3421 catgaatagg cctacgccga gactggcgca gagaagccag cgttggccgt cgtcgaagga
     3481 tggacttccg tcttcgctgc tggtggtcgc ccttttctcg ctagtggtgg tggtgctgct
     3541 ggtgttgctg tcataggagg atttggcact tgcggccgca acatggccgc cagccaacaa
     3601 actcgccatg agcggtaggt gaagagaaat ccacgtaaac gccctgccaa cgctgtgtct
     3661 gatcgggtgc gtataatcaa tcgcacagtc gttatggaca tacatccaat tcaaacagaa
     3721 ggcaataaca agtgtccaca ccgctctcaa aagcgataga ttaaacccga ctgcggcggg
     3781 cgagccgaca atgatgatat atagaaactc tcccaacgca ataatataga atgcagcgaa
     3841 acggtcgatt tcgtgcgcaa cgtcaacggc tgtggtgtac cgcgcgcgaa gcatctgttt
     3901 cgcgagaggg gagtaacaga acatccatgt cccttcttcc aggacctcag ccacagctgc
     3961 aacggcgatt ttgttgcgga gtgaaacact ctcgaagtag agcgggatgt ataagcacaa
     4021 tgcgacggac gacattacga accacaggcg ttgttgtgcg cggtggtggt agctgctgaa
     4081 tgagtaatca ctagtgaatt cgcggccgcc tgcaggtcga ccatatggga gagctcccaa
     4141 cgcgttggat gcatagcttg agtattctat agtgtcacct aaatagcttg gcgtaatcat
     4201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgatgcgg
     6361 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta
     6421 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6481 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6541 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6601 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6661 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6721 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     6781 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     6841 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     6901 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     6961 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     7021 attgtaatac gactcactat a
//
